gfp rela Search Results


p65 hsf1  (Addgene inc)
94
Addgene inc p65 hsf1
P65 Hsf1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+rela/MS2-P65-HSF1_GFP+(Plasmid+%2361423)/pm39799111-209-9-15
Average 94 stars, based on 1 article reviews
p65 hsf1 - by Bioz Stars, 2026-08
94/100 stars
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nfκb  (Addgene inc)
94
Addgene inc nfκb
Figure 1. Exposure to inflammatory cytokines induces mesenchymal transformation in adult endothelial cells. A, Porcine aortic valve endo thelial <t>cell</t> <t>(PAVEC)</t> mesenchymal transformation and invasion of the collagen matrix after 48-hour exposure to 100 ng/mL interleukin-6 (IL-6), 100 ng/mL tumor necrosis factor-α (TNF-α), or genetic overexpression of nuclear factor-κB <t>(NFκB).</t> B, PAVEC endothelial-to-mes enchymal transformation (EndMT)–related gene expression after 48-hour exposure to 100 ng/mL IL-6 or TNF-α, or genetic overexpression of NFκB. C, PAVEC NFκB nuclear localization quantification. D, Confocal images of control, +100 ng/mL IL-6, and +100 ng/mL TNF-α PAVEC at a 48-hour time point stained for CD31 (red), α-smooth muscle actin (α-SMA; green), and DNA (blue). E, Confocal images of con trol, +100 ng/mL IL-6, and +100 ng/mL TNF-α PAVEC at a 48-hour time point stained for vascular endothelial (VE)-cadherin (red), α-SMA (green), and DNA (blue). F, Confocal images of control, +100 ng/mL IL-6, and +100 ng/mL TNF-α PAVEC at a 48-hour time point stained for NFκB (green) and DNA (blue). G, Western blots for PAVEC and porcine aortic valve endothelial cells exposed to 100 ng/mL TNF-α or transforming growth factor-β1 (TGF-β1) for 48 hours. H, Diseased human aortic valve endothelial cells coexpressing α-SMA and CD31 (on the fibrosa or outflow side and invaded, calcified nodule shown in inset). I, Expanded frame of H. J, Nuclear translocation of NFκB in dis eased human valve endothelial cells. Error bars show ±SEM, n≥3 culture wells. Bars that do not share any letters are significantly different according to a 1-way ANOVA with Tukey posttest (P≤0.05). For EndMT gene expression (B), differences that are significantly different than the control according to an unpaired Student t test are indicated with an *. Differences were considered significant at P≤0.05. Scale bars, 25 μm (D and E) or 10 μm (F). PECAM indicates platelet endothelial cell adhesion molecule; PAEC, porcine aortic endothelial cells; VE- cadherin, vascular endothelial-cadherin; TGF-β1, transforming growth factor-β1.
Nfκb, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+rela/GFP-RelA+(Plasmid+%2323255)/10__1161_slash_atvbaha__112__300504-43-15-16
Average 94 stars, based on 1 article reviews
nfκb - by Bioz Stars, 2026-08
94/100 stars
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p65-gfp construct  (Schmid GmbH)
90
Schmid GmbH p65-gfp construct
Figure 1. Exposure to inflammatory cytokines induces mesenchymal transformation in adult endothelial cells. A, Porcine aortic valve endo thelial <t>cell</t> <t>(PAVEC)</t> mesenchymal transformation and invasion of the collagen matrix after 48-hour exposure to 100 ng/mL interleukin-6 (IL-6), 100 ng/mL tumor necrosis factor-α (TNF-α), or genetic overexpression of nuclear factor-κB <t>(NFκB).</t> B, PAVEC endothelial-to-mes enchymal transformation (EndMT)–related gene expression after 48-hour exposure to 100 ng/mL IL-6 or TNF-α, or genetic overexpression of NFκB. C, PAVEC NFκB nuclear localization quantification. D, Confocal images of control, +100 ng/mL IL-6, and +100 ng/mL TNF-α PAVEC at a 48-hour time point stained for CD31 (red), α-smooth muscle actin (α-SMA; green), and DNA (blue). E, Confocal images of con trol, +100 ng/mL IL-6, and +100 ng/mL TNF-α PAVEC at a 48-hour time point stained for vascular endothelial (VE)-cadherin (red), α-SMA (green), and DNA (blue). F, Confocal images of control, +100 ng/mL IL-6, and +100 ng/mL TNF-α PAVEC at a 48-hour time point stained for NFκB (green) and DNA (blue). G, Western blots for PAVEC and porcine aortic valve endothelial cells exposed to 100 ng/mL TNF-α or transforming growth factor-β1 (TGF-β1) for 48 hours. H, Diseased human aortic valve endothelial cells coexpressing α-SMA and CD31 (on the fibrosa or outflow side and invaded, calcified nodule shown in inset). I, Expanded frame of H. J, Nuclear translocation of NFκB in dis eased human valve endothelial cells. Error bars show ±SEM, n≥3 culture wells. Bars that do not share any letters are significantly different according to a 1-way ANOVA with Tukey posttest (P≤0.05). For EndMT gene expression (B), differences that are significantly different than the control according to an unpaired Student t test are indicated with an *. Differences were considered significant at P≤0.05. Scale bars, 25 μm (D and E) or 10 μm (F). PECAM indicates platelet endothelial cell adhesion molecule; PAEC, porcine aortic endothelial cells; VE- cadherin, vascular endothelial-cadherin; TGF-β1, transforming growth factor-β1.
P65 Gfp Construct, supplied by Schmid GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+rela/p65+gfp+construct/pm19841191-425-4-1
Average 90 stars, based on 1 article reviews
p65-gfp construct - by Bioz Stars, 2026-08
90/100 stars
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p65-gfp  (Johns Hopkins HealthCare)
90
Johns Hopkins HealthCare p65-gfp
Figure 1. Exposure to inflammatory cytokines induces mesenchymal transformation in adult endothelial cells. A, Porcine aortic valve endo thelial <t>cell</t> <t>(PAVEC)</t> mesenchymal transformation and invasion of the collagen matrix after 48-hour exposure to 100 ng/mL interleukin-6 (IL-6), 100 ng/mL tumor necrosis factor-α (TNF-α), or genetic overexpression of nuclear factor-κB <t>(NFκB).</t> B, PAVEC endothelial-to-mes enchymal transformation (EndMT)–related gene expression after 48-hour exposure to 100 ng/mL IL-6 or TNF-α, or genetic overexpression of NFκB. C, PAVEC NFκB nuclear localization quantification. D, Confocal images of control, +100 ng/mL IL-6, and +100 ng/mL TNF-α PAVEC at a 48-hour time point stained for CD31 (red), α-smooth muscle actin (α-SMA; green), and DNA (blue). E, Confocal images of con trol, +100 ng/mL IL-6, and +100 ng/mL TNF-α PAVEC at a 48-hour time point stained for vascular endothelial (VE)-cadherin (red), α-SMA (green), and DNA (blue). F, Confocal images of control, +100 ng/mL IL-6, and +100 ng/mL TNF-α PAVEC at a 48-hour time point stained for NFκB (green) and DNA (blue). G, Western blots for PAVEC and porcine aortic valve endothelial cells exposed to 100 ng/mL TNF-α or transforming growth factor-β1 (TGF-β1) for 48 hours. H, Diseased human aortic valve endothelial cells coexpressing α-SMA and CD31 (on the fibrosa or outflow side and invaded, calcified nodule shown in inset). I, Expanded frame of H. J, Nuclear translocation of NFκB in dis eased human valve endothelial cells. Error bars show ±SEM, n≥3 culture wells. Bars that do not share any letters are significantly different according to a 1-way ANOVA with Tukey posttest (P≤0.05). For EndMT gene expression (B), differences that are significantly different than the control according to an unpaired Student t test are indicated with an *. Differences were considered significant at P≤0.05. Scale bars, 25 μm (D and E) or 10 μm (F). PECAM indicates platelet endothelial cell adhesion molecule; PAEC, porcine aortic endothelial cells; VE- cadherin, vascular endothelial-cadherin; TGF-β1, transforming growth factor-β1.
P65 Gfp, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gfp+rela/p65+gfp/pmc07958565-176-0-13
Average 90 stars, based on 1 article reviews
p65-gfp - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


Figure 1. Exposure to inflammatory cytokines induces mesenchymal transformation in adult endothelial cells. A, Porcine aortic valve endo thelial cell (PAVEC) mesenchymal transformation and invasion of the collagen matrix after 48-hour exposure to 100 ng/mL interleukin-6 (IL-6), 100 ng/mL tumor necrosis factor-α (TNF-α), or genetic overexpression of nuclear factor-κB (NFκB). B, PAVEC endothelial-to-mes enchymal transformation (EndMT)–related gene expression after 48-hour exposure to 100 ng/mL IL-6 or TNF-α, or genetic overexpression of NFκB. C, PAVEC NFκB nuclear localization quantification. D, Confocal images of control, +100 ng/mL IL-6, and +100 ng/mL TNF-α PAVEC at a 48-hour time point stained for CD31 (red), α-smooth muscle actin (α-SMA; green), and DNA (blue). E, Confocal images of con trol, +100 ng/mL IL-6, and +100 ng/mL TNF-α PAVEC at a 48-hour time point stained for vascular endothelial (VE)-cadherin (red), α-SMA (green), and DNA (blue). F, Confocal images of control, +100 ng/mL IL-6, and +100 ng/mL TNF-α PAVEC at a 48-hour time point stained for NFκB (green) and DNA (blue). G, Western blots for PAVEC and porcine aortic valve endothelial cells exposed to 100 ng/mL TNF-α or transforming growth factor-β1 (TGF-β1) for 48 hours. H, Diseased human aortic valve endothelial cells coexpressing α-SMA and CD31 (on the fibrosa or outflow side and invaded, calcified nodule shown in inset). I, Expanded frame of H. J, Nuclear translocation of NFκB in dis eased human valve endothelial cells. Error bars show ±SEM, n≥3 culture wells. Bars that do not share any letters are significantly different according to a 1-way ANOVA with Tukey posttest (P≤0.05). For EndMT gene expression (B), differences that are significantly different than the control according to an unpaired Student t test are indicated with an *. Differences were considered significant at P≤0.05. Scale bars, 25 μm (D and E) or 10 μm (F). PECAM indicates platelet endothelial cell adhesion molecule; PAEC, porcine aortic endothelial cells; VE- cadherin, vascular endothelial-cadherin; TGF-β1, transforming growth factor-β1.

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: Inflammatory Cytokines Promote Mesenchymal Transformation in Embryonic and Adult Valve Endothelial Cells

doi: 10.1161/atvbaha.112.300504

Figure Lengend Snippet: Figure 1. Exposure to inflammatory cytokines induces mesenchymal transformation in adult endothelial cells. A, Porcine aortic valve endo thelial cell (PAVEC) mesenchymal transformation and invasion of the collagen matrix after 48-hour exposure to 100 ng/mL interleukin-6 (IL-6), 100 ng/mL tumor necrosis factor-α (TNF-α), or genetic overexpression of nuclear factor-κB (NFκB). B, PAVEC endothelial-to-mes enchymal transformation (EndMT)–related gene expression after 48-hour exposure to 100 ng/mL IL-6 or TNF-α, or genetic overexpression of NFκB. C, PAVEC NFκB nuclear localization quantification. D, Confocal images of control, +100 ng/mL IL-6, and +100 ng/mL TNF-α PAVEC at a 48-hour time point stained for CD31 (red), α-smooth muscle actin (α-SMA; green), and DNA (blue). E, Confocal images of con trol, +100 ng/mL IL-6, and +100 ng/mL TNF-α PAVEC at a 48-hour time point stained for vascular endothelial (VE)-cadherin (red), α-SMA (green), and DNA (blue). F, Confocal images of control, +100 ng/mL IL-6, and +100 ng/mL TNF-α PAVEC at a 48-hour time point stained for NFκB (green) and DNA (blue). G, Western blots for PAVEC and porcine aortic valve endothelial cells exposed to 100 ng/mL TNF-α or transforming growth factor-β1 (TGF-β1) for 48 hours. H, Diseased human aortic valve endothelial cells coexpressing α-SMA and CD31 (on the fibrosa or outflow side and invaded, calcified nodule shown in inset). I, Expanded frame of H. J, Nuclear translocation of NFκB in dis eased human valve endothelial cells. Error bars show ±SEM, n≥3 culture wells. Bars that do not share any letters are significantly different according to a 1-way ANOVA with Tukey posttest (P≤0.05). For EndMT gene expression (B), differences that are significantly different than the control according to an unpaired Student t test are indicated with an *. Differences were considered significant at P≤0.05. Scale bars, 25 μm (D and E) or 10 μm (F). PECAM indicates platelet endothelial cell adhesion molecule; PAEC, porcine aortic endothelial cells; VE- cadherin, vascular endothelial-cadherin; TGF-β1, transforming growth factor-β1.

Article Snippet: For NFκB transfection, PAVEC were trypsinized and electroporated with plasmid encoding the RelA subunit of NFκB (Addgene plasmid 23255) using Neon transfection system (Invitrogen, Carlsbad, CA) and further cultured in 5% serum, antibiotic-free DMEM for 24 hours.30 The cells were then trypsinized, resuspended in normal media (DMEM with 10% fetal bovine serum and 1% penicillin– streptomycin), and seeded onto gels.

Techniques: Transformation Assay, Over Expression, Gene Expression, Control, Staining, Western Blot, Translocation Assay